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장비 상세조회

단백질 아미노산 서열 분석 시스템
Protein Sequencing System

영문약어 PRO
장비명(한글) 단백질 아미노산 서열 분석 시스템 
기기코드 SB124
연구장비표준분류
기기상태 양호 
단백질 아미노산 서열 분석 시스템

부서 및 장비담당자

부서 및 장비담당자
지역 서울센터 
주소 (02855)
서울특별시 성북구 인촌로 22길 6-7 고려대학교 자연캠퍼스 내 한국기초과학지원연구원 
보유센터 및 부서 서울센터 
장비담당자 남명희, 정주희, 정하영
전화 02-6943-4132, 02-6943-4135, 02-6943-4133 
팩스 02-6943-4109 
메일 nammh@kbsi.re.kr  

Spec 및 활용분야

원리 및 특징
    · 1950년 P. Edman이 개발한 단백질의 화학적 degradation을 자동화한 시스템으로써, 적은 시료를 짧은 시간에 분석할 수 있다. 이 시스템은 단백질 또는 펩타이드 N-말단 아미노산을 derivatization시키고, 이를 화학적으로 polypeptide로부 떼어내며, 떨어져 나온 아미노산을 보다 안정된 형태의 아미노산으로 전환시켜 이를 HPLC로 분석하는 과정으로 이루어져 있다.
구성 및 성능
    ○ 구성 : 본체 (자동 화학반응시스템)
    ○ HPLC system,
    ○ Multiwavelength detector,
    ○ data analysis system
    ○ 단백질 양 : 최소 50 pmole이상
    ○ 단백질 순도 : 90% 이상
    ○ Reading capacity : 최대 50~60residue
활용분야
    ○ 단백질 및 펩타이드의 N-말단 아미노산 서열, 내부서열 및 전체서열 분석
    ○ 유전공학적으로 발현된 단백질의약품 구조 확인
    - 재조합 단백질의 N 말단 구조정확성 확인
    - 재조합 단백질 의약품의 구조 안정성 확인
    - 재조합 단배질 발현후 signal sequence 제거 등 processing 과정의 정확성 확인
    ○ 단백질, 펩타이드 내 disufide bond 위치 규명
    ○ 돌연변이성 단백질의 규명
    ○ 미지 단백질 정체 규명
    ○ 가수분해 효소의 작용 site 규명연구

장비 공지사항


연관논문

The protein truncation caused by fusion of PEP-1 peptide and protective roles of transduced PEP-1-MsrA in skin cells
이태형, 최승희, 김화영
BMB Reports, 2011
Purification and characterization of YFGAP, a GAPDH-related novel antimicrobial peptide, from the skin of yellowfin tuna, Thunnus albacares
Jung-Kil Seo, Min Jeong Lee, Hye-Jin Go, Tae Hyun Park, Nam Gyu Park
FISH & SHELLFISH IMMUNOLOGY, 2012
Purification and antimicrobial function of ubiquitin isolated from the gill of\r\nPacific oyster, Crassostrea gigas
Jung-Kil Seo, Min Jeong Lee, Hye-Jin Go, Gun Do Kim, Hyun Do Jeong, Bo-Hye Nam, Nam Gyu Park
MOLECULAR IMMUNOLOGY, 2012
Purification and characterization of an antimicrobial histone H1-like protein\r\nand its gene from the testes of olive flounder, Paralichthys olivaceus
Bo-Hye Nam, Jung-Kil Seo, Hye-Jin Go, Min Jeong Lee, Young-Ok Kim, Dong-Gyun Kim, Sang-Jun Lee, Nam
FISH & SHELLFISH IMMUNOLOGY, 2012
붕장어 피부로부터 평활근 수축작용하는 신경성 펩타이드의 정제
고혜진, 박남규
한국수산과학회, 2012
가물치(Channa argus)로부터 평활근 수축활성 펩타이드의 정제
고혜진, 박남규
한국수산과학회지, 2012
Purification of vasopressin-related peptide, [Arg8]-vsotocin, from the brain of conger eel (Conger myriaster)
고혜진, 김찬희, 김은정, 김인혜, 박희연, 홍용기, 박남규
한국수산과학회지, 2005
붕장어(conger myriaster)의 뇌로부터 Oxytosin-related peptide, Isotocin의 정제
고혜진, 감찬희, 김은정, 김인혜, 안상현, 손희영, 박진일, 박희연, 윤호동, 박남규
한국수산학회지, 2005
Purification and cDNA cloning of a cecropin-like peptide from the great wax moth, Galleria mellonella.
Kim CH, Lee JH, Kim I, Seo SJ, Son SM, Lee KY, Lee IH.
Mol. cells, 2004
Purification, cDNA cloning and expression of an insect defensin from the great wax moth, Galleria mellonella.
Lee YS, Yun EK, Jang WS, Kim I, Lee JH, Park SY, Ryu KS, Seo SJ, Kim CH, Lee IH.
Insect Mol. Biol., 2004
cDNA cloning of halocidin and a new antimicrobial peptide derived from the N-terminus of Ci-META4.
Jang WS, Kim CH, Kang MS, Chae HJ, Son SM, Seo SJ, Lee IH
Peptides, 2005
Decreased entropy of unfolding increases the temperature of maximum stability : Thermodynamic stability of a thioredoxin from the hyperthermophilic archaeon Methanococcus jannaschii
Duck Yeon Lee, Kyeong-Ae Kim, Key-Sun Kim
Journal of the Korean Magnetic Resonance Society, 2004
Substitution of aspartic acid with glutamic acid increases the unfolding transition temperature of a protein
Duck Yeon Lee, Kyeong-Ae Kim, Key-Sun Kim
Biochemical and Biophysical Research Communications, 2004
Purification and characterization of a serine protease with fibrinolytic activity from the dung beetles, Catharsius molossus
Mi Young Ahn, Bum-Soo Hahn, Kang Sun Ryu, Jin Won Kim, Yeong Shik Kim
Thrombosis research, 2003
Triphenylmethane reductase from Citrobacter sp. strain KCTC 18061P: Purification, characterization, gene cloning, and overexpression of a functional protein in Escherichia coli
Moon-Sun Jang, Young-Mi Lee, Cheorl-Ho Kim, Jae-Heon Lee, Dong-Woo Kang, Seok-Jo Kim, Young-Choon Le
Applied and Environmental Microbiology, 2005
Immobile Artificial Metalloproteinase Containing Both Catalytic and Binding Groups.
Bo-Bin Jang, Kwan-Pyo Lee, dal-Hee Min, Junghun Suh
J. American Chemical Society, 1998
고정화된 인공 금속 단백질 가수분해 효소와 우라늄 인식체
장보빈
서울대학교, 1998
Purification and characterization of acharan sulfate lyases, two novel heparinases, from Bacteroides stercoris HJ-15
Byung-Taek Kim, Sung-Woon Hong, Wan-Seok Kim, Yeong Shik Kim and Dong-Hyun Kim
Eur. J. Biochem., 2001
Purification and Characterization of a Novel Heparinase from Bacteroides stercoris HJ-15
Byung-Taek Kim, Wan-Seok Kim, Yeong Shik Kim, Robert J. Linhardt, and Dong-Hyun Kim
J. Biochem., 2000
Identification of Glyceraldehyde-3-phosphate Dehydrogenase by Protein Sequencing in the Rat Postsynaptic Density Fraction
Jung-Il Moon, Jung Dae Kim, Yong Wook Jung, Bok Hyun Ko, Ingnyol Jin, Il Soo Moon
Molecules and Cells,, 1998
Presence of a Voltage-Dependent Anion Channel 1 in the Rat Postsynaptic Density Fraction
Jung-Il Moon, Yong Wook Jung, Bok Hyun Ko, Vito De Pinto, Ingnyol Jin, Il Soo Moon
NeuroReport, 1999
내염성 식물 칠면초(Suaeda japonica Makino)에서 염 스트레스와 관련된 단백질과 색소의 확인 및 특성화
김영철
목원대학교, 1998
Evaluation of Musk by Enzyme-Linked Immunosorbent Assay
Ahn, K.S., Hahn, B.S., Chang, S.Y., Lee, H.K., Jeong, C.S., Kim, Y.S
Biol. Pharm. Bull., 2002
Halocidin: a new antimicrobial peptide from hemocytes of the solitary tunicate, Halocynthia aurantium.
Jang WS, Kim KN, Lee YS, Nam MH, Lee IH.
FEBS Lett., 2002
Comparative study on characteristics of lysozymes from the hemolymph of three lepidopteran larvae, Galleria mellonella, Bombyx mori, Agrius convolvuli.
Yu KH, Kim KN, Lee JH, Lee HS, Kim SH, Cho KY, Nam MH, Lee IH.
Dev. Comp. Immunol., 2002
The solution structure of FADD death domain: structural basis of death domain interaction of fas and FADD
Eui-Jun Jeong, SookHee Bang, Tae Ho Lee, Young In Park, Woong-Seop Sim, Key-Sun Kim
Journal of Biological Chemistry, 1999
Structural analysis of multifunctional peptide motifs in human bifunctional tRNA synthetase: identification of RNA-binding residues and functional implications for tandem repeats
Eui-Jun Jeong, Geum-Sook Hwang, Kyung Hee Kim, Min Jung Kim, Sunghoon Kim, Key-Sun Kim
Biochemistry, 2000
Identification and characterization of buckwheat major allergens
Jung Won Park, Dae Bong Kang, Cheol Woo Kim, Si Hwan Ko, Hye Yung Yum, Kyu Earn Kim, Chein-Soo Hong
Allergy, 2000
Characterization and immunological analysis of ferritin from the hemolymph of Galleria mellonella
Beom Su Kim, Chang Seok Lee, Chi Young Yun, Sung Moon Yeo, Won Mock Park and Hak R. Kim
Comparative Biochemistry and Physiology (A), 2001
Purification and Molecular Cloning of a Thrombin-like Enzyme from Agkstrodon caliginosus (The Korean Viper)
Han B.S., Chang,I.M., Kim,Y.S
J.Biochem. (Tokyo) , 1996
Purification and characterization of fibirinolytic protease from Tenodera sinensis
Hahn, B.S. Jo S.Y, Kim, Y.S
Biochem. Biophys. Acta, 1999
Purification and characterization of a novel serine protease with fibrinolytic activity from Tenodera sinensis (Chinese Mantis)
Cho S.Y. Hahn, B.S., Kim, Y.S.
J. Biochem. Mol. Biol, 1999
Purification and characteization of calobin II, a second thrombin-like enzyme from Agkistrodon caliginosus (Korean viper).
Cho, S.Y. Hanhn, B.S. Yang, K.Y. and Kim, Y.S.
Toxicon, 2001
Purification and characterization of a plasmin-like protease from Tenodera sinsensis (Chinise mantis).
Hahn, B.S., Cho, S.Y. Kim, Y.S.
Insect Biochem. Mol. Biol., 2001
Localization and characterization of acharan sulfate in the body of the giant African snail Achatina fulica.
Jeong, J.A. Toida, T., Linhardt, R., Kim, Y.S.
Comp. Biochem. Physiol., 2001
Purification and Characterization of Apolipophorin-III in the Hemolymph of the Wax Moth, Galleria mellonella L.
Su Jin Lee, In Hee Lee, Chang Soo Kang, Chung Sik Choi, Hwa Kyung Yun
Korean J. Biol Sci., 1997
Purification and Characterization of antibacterial Peptides, Spodopsin Ia and Ib Induced in the Larval Haemolymph of the Common Cutworm, Spodoptera litura
Chung Sik Choi, Sung Moon Yoe, Eung Seok Kim, Kwon Seok Chae, Hak R. Kim
Korean J. Biol Sci., 1997
Purification, Characterization and cDNA Sequence of Apolipophorin-III from the Common Cutworm, Spodoptera litura.
Eung Seok Kim
고려대학교, 1997
담배거세미나방(Spodoptera litura)으로부터 항균단백질 spodopsin Ia, Ib의 정제 및 cDNA sequences
최충식
고려대학교, 1996
미국흰불나방(Hyphantria cunea)의 리포포린과 아포리포포린-III의 정제 및 특성
윤화경
고려대학교, 1994
Purification and Characterization of Insecticyanin from the Last Instar Larval Hemolymph of the Agrius convolvuli
Bo-Young Lee
고려대학교, 1998
미국흰불나방(Hyphantria cunea D.)의 저장단백질-2의 정제 및 특성
나정희
고려대학교, 1994
cDNA Cloning and Antibacterial Activities of Cecropin D-like Peptides from Agrius convolvuli
In Hee Lee, Chung Ryul Kim, Yong Ho Lee, In Seok Bang, Eung Seok Kim, Chang Soo Kang, Chi Young Yu
Archives of insect Biochemistry and Physiology, 2000
Organic Artificial Proteinase with Active Site Comprising Three Salicylate Residues
Junghun Shu, sang Soo Hah
J. American Chemical Society, 1998
Extracellular secretion of a Maltogenic amylase from lactobacillus gasseri ATCC33323 in Lactococcus lactis MG1363 and its application on the production of brached maltooligosaccharides
Cho, MH; Park, SE; Lee, MH; Ha, SJ; Kim, HY; Kim, MJ; Lee, SJ; Madsen, SM; Park, CS
Journal of microbiology and biotechnology, 2007
보통 밀에서 저분자글루테닌 유전자 클로닝 및 단백질 동정
이종렬
한국육종학회, 2010
Extracellular Gelatinase of Enterococcus faecalis Destroys a Defense System in Insect Hemolymph and Human Serum
박신용, 김경미, 이준하, 서숙재, 이인희
INFECTION AND IMMUNITY (IAI), 2007
Antimicrobial Effect of Halocidin-Derived Peptide in a Mouse Model of Listeria Infection
장웅식, 이상철, 이영신, 신용표, 신경화, 송부희, 김병삼, 이수한, 이인희
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007
Autocatalytic processing of HtrA2/Omi is essential for induction of caspase-dependent cell death through antagonizing XIAP.
Seong YM, Choi JY, Park HJ, Kim KJ, Ahn SG, Seong GH, Kim IK, Kang S, Rhim H.
Journal of Biological Chemistry, 2004
Beta-amyloid precursor protein is a direct cleavage target of HtrA2 serine protease. Implications for the physiological function of HtrA2 in the mitochondria.
Park HJ, Kim SS, Seong YM, Kim KH, Goo HG, Yoon EJ, Min do S, Kang S, Rhim H.
Journal of Biological Chemistry, 2006

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