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Spec 및 활용분야

원리 및 특징	· 분자량이 비교적 큰 시료와 매트릭스가 혼합된 결정에 순간적으로 레이저를 조사하여 이온화시킨 후, 이온들을 비행시간형 질량분석기를 통과시켜 검출기까지의 도달시간을 측정하여 분자량을 얻는다. · High Mass detector 인 CovalX 를 이용하여 고분자량 물질의 질량 분석이 가능하다.
구성 및 성능	○TOF mass range (Using High Mass Detector) - ≤ 1,500 kDa ○Mass resolution - Linear TOF mode (Protein) ≥ 700 for Insulin (m/z 5,734) FWHM, ≥ 1,000 for Myoglobin M2+ (m/z 8,476) FWHM ≥ 1,200 for Cytochrome C (m/z 12,361) FWHM, ≥ 1,100 for Myoglobin (m/z 16,952) FWHM - Reflector TOF mode ≥ 40,000 for Somatostatin 28 (m/z 3,147.47) FWHM - MS/MS mode: ≥ 1,500 for [Glu1]-Fib fragment m/z 175, ≥ 2,500 for [Glu1]-Fib fragment m/z 684 ≥ 3,500 for [Glu1]-Fib fragment m/z 1,056, ≥ 4,500 for [Glu1]-Fib fragment m/z 1,441 ○Max. data aquisition speed - 1 kHz ○Mass accuracy (Protein mixture) - MS mode: better than 60 ppm (With external calibrant), better than 50 ppm (With internal calibrant) - MS/MS mode: Average Mass Error ≤ 0.05 Da for relevant fragments of [Glu1]-Fibrinopeptide B (m/z 1,570) ○Mass sensitivity - Single-MS mode: 500 fmol BSA (m/z 66,000) at S/N ≥ 100:1 shown with 1,000 laser shots - MS-MS mode: 250 amol [Glu1]-Fibrinopeptide B fragment 1,056 Da at S/N ≥ 20:1 ○Ionization - BRUKER smartbeam II laser, 355 nm wavelength
활용분야	■시료의 분자량 측정 및 단백질 동정 ○ 단백질, 펩타이드, 합성물질, 폴리머, DNA 등의 다양한 시료의 분자량 측정 ○ High mass detector 를 이용한 ~1,500 kD 의 고분자 단백질의 분자량 측정 ○ 바이오시밀러 분석, 기능성 프로테옴 분석 ○ 펩타이드의 ms 및 ms/ms 측정을 통한 단백질 동정 ○ Ribosomal protein mapping 을 통한 미생물 분류 ○ 단백질 결합 및 단백질 복합체 분석, 항원 항체 결합 및 protein aggregates 분석 ○ protein (peptide) and compound 분자량 측정 ○ peptide sequencing을 통한 단백질 동정 ○ pI 및 분자량에 따른 단백질 분리 후 각 2.D spot의 동정

Synthesis of Poly(benzyl ether) Dendrimers Containing Core Diversities by Click Chemistry

이재욱(Jae Wook Lee)

Bull. Korean Chem. Soc., 2009

Facile Synthesis of Poly(benzyl ether) Dendrimers by Click Chemistry

이재욱(Jae Wook Lee)

Polymer (Korea), 2009

Staudinger/Aza-Wittig Click Chemistry for Synthesis of Dendrimers using Fréchet Type Dendritic Benzyl Azides

이재욱(Jae Wook Lee)

Bull. Korean Chem. Soc., 2009

Facile Synthesis of Dendritic-Linear-Dendritic Materials by Click Chemistry

이재욱(Jae Wook Lee)

Macromol. Res., 2009

Synthesis of Dendrimers by Homocoupling of Alkyne-focal Fréchet Type Dendrons

이재욱(Jae Wook Lee)

Bull. Korean Chem. Soc., 2009

Oleanane-Type Triterpenoids from Aceriphyllum rossii and Their Cytotoxic Activity

Le Thi Kim Van, Tran Manh Hung, Phuong Thien Thuong, Tran Minh Ngoc, Jin Cheol Kim, Han-Su Jang, Xin

JOURNAL OF NATURAL PRODUCTS, 2009

Regulation of retinal axon growth by secreted Vax1 homeodomain protein.

Kim N, Min KW, Kang KH, Lee EJ, Kim HT, Moon K, Choi J, Le D, Lee SH, Kim JW.

eLife, 2014

Dinuclear Iridium(III) Complexes Linked by a Bis(β-diketonato) Bridging Ligand: Energy Convergence versus Aggregation-Induced Emission

Chang Hwan Shin,[a] Jung Oh Huh,[a] Sun Jong Baek,[a] Sang Kyu Kim,[a]

EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2010

Vinyl-Type Polynorbornenes with Pendant PCBM: A Novel Acceptor for Organic Solar Cells

Maengsun Eo, Soohyun Lee, Myung Hwan Park, Min Hyung Lee,*

MACROMOLECULAR RAPID COMMUNICATIONS, 2012

Synthesis of Dendrimers from Alkyne-focal Dendrons by Oxidative Homo-coupling of Terminal Acetylene

Jae Wook Lee

Bull. Korean Chem. Soc., 2011

Synthesis of Dendrimer Containing Dialkylated-fluorene Unit as a Core Chromophore via Click Chemistry

한승철, 진성호, 이재욱

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2012

Synthesis of Unsymmetrical Fréchet-type Dendrimers via Double Click Chemistry

한승철, 곽승환, 진성호, 이재욱

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2012

Synthesis of Dendrimers via Staudinger/aza-Wittig Reactions of Fréchet-type Dendritic Benzyl Azides and Fréchet-type Dendritic Benzaldehydes

한승철, 이재욱, 진성호

MACROMOLECULAR RESEARCH, 2012

Synthesis of Dendrimer with PEG core by Click Chemistry

한승철, 진성호, 이재욱

POLYMER-KOREA, 2012

Epidermal Growth Factor-Like Domain Repeat of Stabilin-2 Recognizes Phosphatidylserine during Cell Corpse Clearance

Seung-Yoon Park, So-Youn Kim, Mi-Yeon Jung, Dong-Jun Bae, and In-San Kim

MOLECULAR AND CELLULAR BIOLOGY, 2008

Proteomics-based dissection of biotic stress responsive proteins in bread wheat (Triticum aestivum L.)

Abu Hena Mostafa Kamal, Ki-Hyun Kim, Jong-Soon Choi, Hisashi Hirano, Hwa-Young Heo, Sun-Hee Woo

African Journal of Biotechnology, 2010

Reference proteome map of buckwheat (Fagopyrum esculentum and Fagopyrum tataricum) leaf and stem cultured under light or dark

Dong-Hoon Shin, Abu Hena Mostafa Kamal, Tatsuro Suzuki, Moon-Soon Lee,Jong-Soon Choi, Sun-Hee Woo

Australian Journal of Crop Science, 2010

Platelet factor-4 is an indicator of blood count recovery in acute myeloid leukemia patients in complete remission.

JY Kim, HJ Song, HJ Lim, MG Shin, JS Kim, HJ Kim, BY Kim, and SW Lee*

Molecular & Cellular Proteomics, 2008

Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate)

Han, M.J., Yoon, S.S., and Lee, S.Y.

J. Bacteriol., 2001

Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture

Yoon,S.H., Han, M.J., Lee, S.Y., Jeong, K.J., and Yoo, J.S.

Biotechnol. Bioeng., 2003

Engineering Escherichia coli for increased production of serine-rich proteins based on proteome profiling

Han, M.J., Jeong, K.J., Yoo, J.S., and Lee. S.Y.

Appl. Environ. Microbiol., 2003

Micropatterns of spores displaying heterologous proteins

Park, T.J., Lee, K.-B., Lee, S.J., Park, J.P., Lee, Z.-W., Lee, S.Y. and Choi, I.S.

J. Am. Chem. Soc., 2004

Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner

Park, J.P. et al.

Biotechnol. Bioeng., 2005

Analysis of poly(3-hydroxybutyrate) granule-associated proteome in recombinant Escherichia coli

Han, M.-J., Park, S.J., Lee, J.W., Min, B.H., Lee, S.Y., Kim, S.-J., and Yoo, J.-S.

J. Microbiol. Biotechnol., 2006

The proteome of Mannheimia succiniciproducens, a capnophilic rumen bacterium

Lee, J.W., Lee, S.Y., Song, H., and Yoo, J.S.

Proteomics, 2006

Protein nanopatterns and biosensors using gold binding polypeptide as a fusion partner

Park, T.J. et al.

Anal. Chem., 2008

Proteome-level responses of Escherichia coli to long-chain fatty acids and use of fatty acid inducible promoter in protein production

Han, M.-J., Lee, J.W., Lee, S.Y. and Yoo, J.

J. Biomedicine Biotechnol., 2008

Comparison of the extracellular proteomes of Escherichia coli B and K-12 strains during high cell density cultivation

Xia, X.-X., Han, M.-J., Lee, S.Y., and Yoo, J.-S.

Proteomics, 2008

Development of a fully integrated microfluidic system for sensing infectious viral disease

Huh, Y.S., Park, T.J., Lee, E.Z., Hong, W.H., and Lee, S.Y.

Electrophoresis, 2008

Synthesis and characterization of mononuclear and dinuclear Mn complexes with N,N0-disubstituted octaaza macrocycle

Wonchoul Park a, Jang-Hoon Cho, Hong-In Lee, Mira Park, Myoung Soo Lah, Dongyeol Lim

Polyhedron, 2008

Biophysical Studies Reveal Key Interactions between Papiliocin Derived PapN and Lipopolysaccharide in Gram-Negative Bacteria

Prasannavenkatesh Durai, Yeongjoon Lee, Jieun Kim, Dasom Jeon, and Yangmee Kim

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2018

Excimer Emission-ba&#38ltx&#38gtsed Fluorescent Probe Targeting Caspase‑3

Tae-Il Kim, Hanyong Jin, Jeehyeon Bae, Youngmi Kim

ANALYTICAL CHEMISTRY, 2017

A novel method to rapidly distinguish the geographical origin of traditional fermented-salted vegetables by mass fingerprinting

윤소라, 김성현, 이해원, 하지형

PLoS One, 2017

Enhancing bacterial production of a recombinant cetuximab-Fab by partial humanization and its utility for drug conjugation

Dae WonSim,JinsueSong,Ji HunKim,Jun KyoungLee,Da YoonChung,Ku SungJo,Chan GilKim,Min DukSeo,Ho ChulKang,Jin ChulPaeng,Young PilKim,Young PilKim,Hyung SikWon

PROCESS BIOCHEMISTRY, 2018

Phloretin protects macrophages from E. Coli-Induced Inflammation through the TLR4 Signaling Pathway

Anil KumarChauhan, MiheeJang, YangmeeKim,

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2020

Isorhamnetin Has Potential for the Treatment of Escherichia coli-Induced Sepsis.

Anil Kumar Chauhan, Jieun Kim, Yeongjoon Lee, Pavithra K. Balasubramanian and Yangmee Kim

MOLECULES, 2019

Visible-Light-Induced Cysteine-Specific Bioconjugation: Biocompatible Thiol–Ene Click Chemistry

HangyeolChoi, MyojeongKim, JaebongJang, SungwooHong

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2020

Effects of C-Terminal Residues of 12-Mer Peptides on Antibacterial Efficacy and Mechanism

Kkabi Son, Jieun Kim, Mihee Jang, Anil Kumar Chauhan and Yangmee Kim

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2019

Anti-endotoxin 9-meric peptide with therapeutic potential for the treatment of endotoxemia

ManigandanKrishnan, JoonhyeokChoi, SungjaeChoi, YangmeeKim,

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2021

A novel peptide antibiotic, pro10-1d, designed from insect defensin shows antibacterial and anti-inflammatory activities in sepsis models

ManigandanKrishnan, JoonhyeokChoi, AhjinJang, YangmeeKim,

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020

Antiseptic Effect of Ps-K18: Mechanism of Its Antibacterial and Anti-Inflammatory Activities.

Mihee Jang, Jieun Kim, Yujin Choi, JeongKyu Bang and Yangmee Kim

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019

Development of Novel Peptides for the Antimicrobial Combination Therapy against Carbapenem-Resistant Acinetobacter baumannii Infection

Joonhyeok Choi, Ahjin Jang, Young Kyung Yoon, Yangmee Kim

Pharmaceutics, 2021

Highly luminescent and catalytically active suprastructures of magic-sized semiconductor nanoclusters

WoonhyukBaek, Megalamane S.Bootharaju, Kelly M.Walsh, SanghwaLee, Daniel R.Gamelin, TaeghwanHyeon,

NATURE MATERIALS, 2021