KBSI 연구장비공동활용 장비상세조회
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기기검색

장비 상세조회

매트릭스 보조 레이저 탈착 이온화 비행시간 질량분석 시스템
Matrix-Assisted Laser Desorption Ionization Mass Spectrometer system

분석장비 상세내역
영문약어 MALDI TOF/TOF MS
장비명(한글) 매트릭스 보조 레이저 탈착 이온화 비행시간 질량분석 시스템 
기기코드 AS42
연구장비표준분류
기기상태  
AS42.jpg

부서 및 장비담당자

부서 및 장비담당자
지역 오창본원 
주소  
보유센터 및 부서 오창본원 질환표적기능연구팀 
장비담당자 정영호  
전화   ◀ 전화기 아이콘을 누르시면 전화번호를 볼 수 있습니다.
팩스 043-240-5158 
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Spec 및 활용분야

Spec 및 활용분야
원리 및 특징
    · 분자량이 비교적 큰 시료와 매트릭스가 혼합된 결정에 순간적으로 레이저를 조사하여 이온화시킨 후, 이온들을 비행시간형 질량분석기를 통과시켜 검출기까지의 도달시간을 측정하여 분자량을 얻는다.

    · High Mass detector 인 CovalX 를 이용하여 고분자량 물질의 질량 분석이 가능하다.
구성 및 성능
    ○TOF mass range (Using High Mass Detector)
    - ≤ 1,500 kDa
    ○Mass resolution
    - Linear TOF mode (Protein)
    ≥ 700 for Insulin (m/z 5,734) FWHM, ≥ 1,000 for Myoglobin M2+ (m/z 8,476) FWHM
    ≥ 1,200 for Cytochrome C (m/z 12,361) FWHM, ≥ 1,100 for Myoglobin (m/z 16,952) FWHM
    - Reflector TOF mode
    ≥ 40,000 for Somatostatin 28 (m/z 3,147.47) FWHM
    - MS/MS mode:
    ≥ 1,500 for [Glu1]-Fib fragment m/z 175, ≥ 2,500 for [Glu1]-Fib fragment m/z 684
    ≥ 3,500 for [Glu1]-Fib fragment m/z 1,056, ≥ 4,500 for [Glu1]-Fib fragment m/z 1,441
    ○Max. data aquisition speed
    - 1 kHz
    ○Mass accuracy (Protein mixture)
    - MS mode: better than 60 ppm (With external calibrant), better than 50 ppm (With internal calibrant)
    - MS/MS mode: Average Mass Error ≤ 0.05 Da for relevant fragments of [Glu1]-Fibrinopeptide B (m/z 1,570)
    ○Mass sensitivity
    - Single-MS mode: 500 fmol BSA (m/z 66,000) at S/N ≥ 100:1 shown with 1,000 laser shots
    - MS-MS mode: 250 amol [Glu1]-Fibrinopeptide B fragment 1,056 Da at S/N ≥ 20:1
    ○Ionization
    - BRUKER smartbeam II laser, 355 nm wavelength
활용분야
    ■시료의 분자량 측정 및 단백질 동정
    ○ 단백질, 펩타이드, 합성물질, 폴리머, DNA 등의 다양한 시료의 분자량 측정
    ○ High mass detector 를 이용한 ~1,500 kD 의 고분자 단백질의 분자량 측정
    ○ 바이오시밀러 분석, 기능성 프로테옴 분석
    ○ 펩타이드의 ms 및 ms/ms 측정을 통한 단백질 동정
    ○ Ribosomal protein mapping 을 통한 미생물 분류
    ○ 단백질 결합 및 단백질 복합체 분석, 항원 항체 결합 및 protein aggregates 분석
    ○ protein (peptide) and compound 분자량 측정
    ○ peptide sequencing을 통한 단백질 동정
    ○ pI 및 분자량에 따른 단백질 분리 후 각 2.D spot의 동정

장비 공지사항


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Regulation of retinal axon growth by secreted Vax1 homeodomain protein.
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Dinuclear Iridium(III) Complexes Linked by a Bis(β-diketonato) Bridging Ligand: Energy Convergence versus Aggregation-Induced Emission
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Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate)
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Combined transcriptome and proteome analysis of Escherichia coli during high cell density culture
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Engineering Escherichia coli for increased production of serine-rich proteins based on proteome profiling
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Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner
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이용자 리뷰


분석지원 상담센터 분석지원 상담센터
분석지원 상담센터 - 상담분야, ARS, 상태 항목으로 구분되어 있습니다.
상담분야 ARS 상태
바이오 분야

1

재실
소재 분야

2

부재
환경 분야

3

부재
총괄/기타

4

재실